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biotinylated horse anti goat secondary antibody for pdgfrα  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated horse anti goat secondary antibody for pdgfrα
    Representative micrographs of A) NogoA immunoreactive OLs and B) <t>PDGFRα</t> immunoreactive OPCs. Scale bar = 10 µm. Images were published in Dr. John Kim’s thesis . Bar plots showing groups means ± standard error of the mean for C) OL soma volume and D) OPC soma volume. Bar plots showing groups means ± standard error of the mean for E) OL density and F) OPC density. Scatter plots with trend line showing the relationships between age and G) OL density and H) OPC density. Magenta trend line denotes positive model coefficient for age while blue trend line denotes negative model coefficient for age. Only the age and OL density relationship was statistically significant (p = 0.010).
    Biotinylated Horse Anti Goat Secondary Antibody For Pdgfrα, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1683 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+horse+anti+goat+igg+secondary+antibody/Biotinylated+Horse+Anti-Goat+IgG+Antibody/bio_rxiv__64898__2026__03__05__708309-53-24-31
    Average 96 stars, based on 1683 article reviews
    biotinylated horse anti goat secondary antibody for pdgfrα - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "A multimodal characterization of the human uncinate fasciculus"

    Article Title: A multimodal characterization of the human uncinate fasciculus

    Journal: bioRxiv

    doi: 10.64898/2026.03.05.708309

    Representative micrographs of A) NogoA immunoreactive OLs and B) PDGFRα immunoreactive OPCs. Scale bar = 10 µm. Images were published in Dr. John Kim’s thesis . Bar plots showing groups means ± standard error of the mean for C) OL soma volume and D) OPC soma volume. Bar plots showing groups means ± standard error of the mean for E) OL density and F) OPC density. Scatter plots with trend line showing the relationships between age and G) OL density and H) OPC density. Magenta trend line denotes positive model coefficient for age while blue trend line denotes negative model coefficient for age. Only the age and OL density relationship was statistically significant (p = 0.010).
    Figure Legend Snippet: Representative micrographs of A) NogoA immunoreactive OLs and B) PDGFRα immunoreactive OPCs. Scale bar = 10 µm. Images were published in Dr. John Kim’s thesis . Bar plots showing groups means ± standard error of the mean for C) OL soma volume and D) OPC soma volume. Bar plots showing groups means ± standard error of the mean for E) OL density and F) OPC density. Scatter plots with trend line showing the relationships between age and G) OL density and H) OPC density. Magenta trend line denotes positive model coefficient for age while blue trend line denotes negative model coefficient for age. Only the age and OL density relationship was statistically significant (p = 0.010).

    Techniques Used:

    Related Articles

    Incubation:

    Article Title: Exaggerated postnatal surge of orexin and the effects of elimination of excess orexin on blood pressure in spontaneously hypertensive rats in postnatal development
    Article Snippet: .. Brain sections were then incubated in a goat polyclonal anti-OXA primary antibody (1:10,000 dilution; SC-8070, Santa Cruz, Dallas, TX, USA) followed by biotinylated horse anti-goat IgG secondary antibody (1:1,000 dilution; Vector Laboratories, Burlingame, CA, USA). ..

    Article Title: Prediction of melanoma metastasis by the Shields index based on lymphatic vessel density
    Article Snippet: Paraffin embedded sections were dewaxed re-hydrated, and microwave antigen retrieval carried out in 0.01 M sodium citrate, pH 6.0, 10 minutes at 800 W incubated in 3% hydrogen peroxide for 5 minutes, washed twice in phosphate buffered saline (PBS (mM): NaCl, 137; KCl, 2.68; Na2HPO4, 10; KH2PO4, 1.76), incubated in 1.5% normal horse serum in 1 × PBS for 30 minutes, and overnight at 4°C in a humid chamber with polyclonal goat anti-human LYVE-1 antibody; 15 μg/ml (AF2089, R&D Systems, UK) or normal goat IgG (1-5000, Vector Laboratories, Peterborough, UK), in non-immune block (Zymed Laboratories, San Fransisco, USA). .. Slides were washed twice in PBS/Tween, 0.05% v/v, (PBT) before blocking and incubation with biotinylated horse anti-goat IgG secondary antibody (2 μg/ml BA-9500, Vector Laboratories, Peterborough, UK) in blocking solution, in a humid chamber for 30 minutes. .. Sections were washed twice with 1 × PBT for 5 minutes before incubating for 30 minutes with Elite ABC Kit, (Vector Laboratories, Peterborough, UK) at room temperature.

    Article Title: Lethal eosinophilic crystalline pneumonia in mice expressing a stabilized Csf2 mRNA
    Article Snippet: Non-specific sites were blocked with avidin-biotin blocking reagents for Ym1 and F4/80 immunostaining (Vector Laboratories, Burlingame, CA) or Rodent Block M for SP-A immunostaining (Biocare Medical). .. Sections were incubated either with goat anti-mouse Ym1 (Chitinase 3-like 3/ECF-L) antibody (AF2446, R&D Systems, Minneapolis, MN) at a dilution of 1:500, followed by biotinylated anti-goat IgG (H+L) secondary antibody (1:2000; Vector Laboratories), rat anti-mouse F4/80 monoclonal antibody (BM8; 123102, BioLegend San Diego, CA) at a dilution of 1:500 followed by biotinylated rabbit anti-rat IgG secondary antibody (1:500; Vector Laboratories), or goat polyclonal SP-A antibody (sc-7699, Santa Cruz Biotechnology, Dallas, TX) at a dilution of 1:100 followed by biotinylated horse anti-goat IgG secondary antibody (1:500; Vector Laboratories). ..

    Article Title: Stereological analysis of cholinergic neurons within bilateral pedunculopontine nuclei in health and when affected by Parkinson's disease
    Article Snippet: The sections were then incubated overnight at 4°C with the goat polyclonal primary antibody (1:300, #AB144P, Millipore) for labelling cholinergic neurons by recognizing choline acetyltransferase (ChAT), the enzyme that catalyzes the resynthesis of acetylcholine (ACh). .. After overnight incubation, the samples were washed well with PBS before applying biotinylated horse (anti‐goat) IgG secondary antibody (1:200, #BA‐9500, Vector Laboratories) for 2 h at RT, and then washing with PBS. .. The samples were incubated in avidin‐biotin complex solution (Vector Laboratories, UK) for 30 min at RT, washed with PBS and then applying DAB (3,3′‐diaminobenzidine; Vector Laboratories) chromogen for ~10 min to visualize the immunoreaction.

    Article Title: Doublecortin-expressing cells are selectively altered in the piriform cortex but not in neurogenic areas of symptomatic Mecp2-heterozygous mice.
    Article Snippet: Brain slices were then incubated overnight at 4 ◦C with a goat primary antibody anti-DCX (C-18, sc:8066, Santa Cruz Biotechnology, Inc) diluted 1:500 in 0.05 M TBS pH 7.4, with 2 % NHS and 0.3 % Triton-X100. .. The next day, sections were incubated for two hours at RT with biotinylated horse anti-Goat-IgG secondary antibody (BA-9500, Vector) diluted 1:200 in the same buffer. .. Afterwards, sections were incubated for 90 min at RT in ABC (Vector) in TBS with 0.3 % Triton-X100.

    Blocking Assay:

    Article Title: Prediction of melanoma metastasis by the Shields index based on lymphatic vessel density
    Article Snippet: Paraffin embedded sections were dewaxed re-hydrated, and microwave antigen retrieval carried out in 0.01 M sodium citrate, pH 6.0, 10 minutes at 800 W incubated in 3% hydrogen peroxide for 5 minutes, washed twice in phosphate buffered saline (PBS (mM): NaCl, 137; KCl, 2.68; Na2HPO4, 10; KH2PO4, 1.76), incubated in 1.5% normal horse serum in 1 × PBS for 30 minutes, and overnight at 4°C in a humid chamber with polyclonal goat anti-human LYVE-1 antibody; 15 μg/ml (AF2089, R&D Systems, UK) or normal goat IgG (1-5000, Vector Laboratories, Peterborough, UK), in non-immune block (Zymed Laboratories, San Fransisco, USA). .. Slides were washed twice in PBS/Tween, 0.05% v/v, (PBT) before blocking and incubation with biotinylated horse anti-goat IgG secondary antibody (2 μg/ml BA-9500, Vector Laboratories, Peterborough, UK) in blocking solution, in a humid chamber for 30 minutes. .. Sections were washed twice with 1 × PBT for 5 minutes before incubating for 30 minutes with Elite ABC Kit, (Vector Laboratories, Peterborough, UK) at room temperature.

    Immunohistochemistry:

    Article Title: LGALS3BP antibody-drug conjugate enhances tumor-infiltrating lymphocytes and synergizes with immunotherapy to restrain neuroblastoma growth.
    Article Snippet: .. The biotinylated horse anti-goat IgG secondary antibody (BA-9500, Vector Laboratories) and the HRP Detection IHC kit (ab93677, Abcam) were used to detect the antigen. .. After incubation with the chromogen 3,3-diaminobenzidine DAB (K3468, Dako), slides were counterstained in Hematoxylin (CATHE-MM, Biocare Medical) and scanned with Nanozoomer scanner from Hamamatsu.

    Article Title: LGALS3BP antibody-drug conjugate enhances tumor-infiltrating lymphocytes and synergizes with immunotherapy to restrain neuroblastoma growth
    Article Snippet: .. The biotinylated horse anti-goat IgG secondary antibody (BA-9500, Vector Laboratories) and the HRP Detection IHC kit (ab93677, Abcam) were used to detect the antigen. .. After incubation with the chromogen 3,3-diaminobenzidine DAB (K3468, Dako), slides were counterstained in Hematoxylin (CATHE-MM, Biocare Medical) and scanned with Nanozoomer scanner from Hamamatsu.



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    Representative micrographs of A) NogoA immunoreactive OLs and B) <t>PDGFRα</t> immunoreactive OPCs. Scale bar = 10 µm. Images were published in Dr. John Kim’s thesis . Bar plots showing groups means ± standard error of the mean for C) OL soma volume and D) OPC soma volume. Bar plots showing groups means ± standard error of the mean for E) OL density and F) OPC density. Scatter plots with trend line showing the relationships between age and G) OL density and H) OPC density. Magenta trend line denotes positive model coefficient for age while blue trend line denotes negative model coefficient for age. Only the age and OL density relationship was statistically significant (p = 0.010).
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    Image Search Results


    Representative micrographs of A) NogoA immunoreactive OLs and B) PDGFRα immunoreactive OPCs. Scale bar = 10 µm. Images were published in Dr. John Kim’s thesis . Bar plots showing groups means ± standard error of the mean for C) OL soma volume and D) OPC soma volume. Bar plots showing groups means ± standard error of the mean for E) OL density and F) OPC density. Scatter plots with trend line showing the relationships between age and G) OL density and H) OPC density. Magenta trend line denotes positive model coefficient for age while blue trend line denotes negative model coefficient for age. Only the age and OL density relationship was statistically significant (p = 0.010).

    Journal: bioRxiv

    Article Title: A multimodal characterization of the human uncinate fasciculus

    doi: 10.64898/2026.03.05.708309

    Figure Lengend Snippet: Representative micrographs of A) NogoA immunoreactive OLs and B) PDGFRα immunoreactive OPCs. Scale bar = 10 µm. Images were published in Dr. John Kim’s thesis . Bar plots showing groups means ± standard error of the mean for C) OL soma volume and D) OPC soma volume. Bar plots showing groups means ± standard error of the mean for E) OL density and F) OPC density. Scatter plots with trend line showing the relationships between age and G) OL density and H) OPC density. Magenta trend line denotes positive model coefficient for age while blue trend line denotes negative model coefficient for age. Only the age and OL density relationship was statistically significant (p = 0.010).

    Article Snippet: After a series of 3 washes in PBS, the tissues were incubated in biotinylated goat anti-rabbit secondary antibody for NogoA (Vector Laboratories BA-1000) and biotinylated horse anti-goat secondary antibody for PDGFRα (Vector Laboratories BA-9500) diluted in blocking buffer for 2 hours at room temperature.

    Techniques: